FAQ: Is BspEI activity sensitive to dam, dcm or mammalian CpG methylation?

Yes. BspEI cannot cut recognition sites that contain overlapping dam methylation. In mammalian gDNA BspEI cannot cut sites that contain CpG methylation. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE.

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