HomeFAQsI've blunted my DNA and now need to dephosphorylate the reaction with Antarctic Phosphatase. Do I need to purify the reaction?
FAQ: I've blunted my DNA and now need to dephosphorylate the reaction with Antarctic Phosphatase. Do I need to purify the reaction?
Yes, the reaction will need to be purified prior to dephosphorylation with Antarctic Phosphatase, CIP or any other commercially available phosphatase. The reaction can be purified using a commercial purification kit, phenol extraction/ethanol precipitation or gel electrophoresis.
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