FAQ: Is FokI blocked by methylation?

FokI will cut 2-4 times slower if the end C in the Sequence is followed by a G and is methylated. Also, FokI will not cut DNA comletely if there is an MspI methylation site before the recognition site. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE.

Links to this resource

Related Products: