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  • FAQ: How much substrate can be phosphorylated in a standard reaction?

    Up to 350 pmol of 5´ termini for cold phosphorylation and up to 50 pmol for hot phosphorylation.
    Note: 1 µg of a 20 mer = 150 pmol of 5´ termini.
    Example, to calculate the number of pmol in 1 µg of a 30 mer: 150 pmol X (20 mer/30 mer) = 100 pmol