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  • FAQ: What amount of growth media from cells expressing GLuc should I use to detect the presence of GLuc by using the Anti-GLuc Antibody in a Western?

    The minimum amount of growth media needed in a Western to detect GLuc activity will vary from cell line to cell line, and will depend on the amount of GLuc expressed by the cells and the transfection efficiency. To start titering the amount of growth media that will be needed to detect GLuc in a Western by using the Anti-GLuc Antibody use a minimum of 0.1% of total growth media (from a high efficiency transfection).