HomeFAQsAre there any special considerations when working with BbvI?
FAQ: Are there any special considerations when working with BbvI?
Like many type IIS (shifted cut site) restriction enzymes, BbvI seems to remain bound to its substrate after cleavage. Extended digests are not recommended because 1 unit is required to cleave 1 μg of DNA in 16 hours. Phenol extraction is the suggested method of DNA purification due to the binding properties of the enzyme.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.