HomeFAQsHow many units of T4 Polynucleotide Kinase should be used for a typical reaction?
FAQ: How many units of T4 Polynucleotide Kinase should be used for a typical reaction?
Typically, 10 units for a cold reaction and 20 units for a hot labeling reaction. Since the [substrate] and the [ATP] are below the Km in a hot reaction, more units are required. The unit determination is done under ideal conditions where the substrate and ATP concentrations are above their Km's.