Single base-pair overhangs are poor substrates for T4 DNA ligase: they anneal very poorly, and are as difficult to ligate as are blunt ended DNA fragments. Also, for AlwI the overhang is not constrained to be any particular nucleotide; all four bases are roughly equally represented. Consequently, any given overhang is compatible with only 25% of the other overhangs present. This lowers ligation efficieny relative to overhangs generated by restriction enzymes which leave only mutually compatible overhangs.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.