HomeFAQsWhen I run my sample in a gel after digesting with BseYI, the DNA doesn’t seem to migrate correctly. Is there a reason for that?
FAQ: When I run my sample in a gel after digesting with BseYI, the DNA doesn’t seem to migrate correctly. Is there a reason for that?
Yes. BseYI can remain bound to the DNA substrate after digestion altering the migration rate of the DNA during electrophoresis. To disrupt this binding, SDS to a final concentration of 0.5% can be added to the loading buffer prior to loading the sample in a gel. Alternatively, the DNA can be purified prior to electrophoresis.
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