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  • FAQ: I want to use Trypsin on 20 ug of protein. How much enzyme do I need to use?

    Use a ratio by weight of 20:1 substrate to Trypsin, therefore, in this case add 10 ul Trypsin stock solution (100 ng) to the reaction. We never exceed a 10:1 ratio of sample:Trypsin. Some users use ratios as low as 100:1 so as to limit Trypsin as a contaminate in the analysis. Care must be taken when using low amounts of Trypsin relative to protein that a complete digestion is still obtained.