FAQ: How does MlyI differ from its neoschizomers, PleI and N.BstNI?

MlyI recognizes GAGTC(N5) and leaves a blunt end. PleI recognizes GAGTC (N4)/(N5) and leaves a one base 5´ overhang. N.BstNI recognizes GAGTC(N4) and nicks one strand.

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