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  • FAQ: How can ligation efficiency be increased?

    Less than 50% of AflII fragments ligate in a standard 20 microliter reaction containing 100-500 units of T4 DNA ligase. Ligation can be enhanced by using 1 microliter of high concentration T4 DNA ligase. Blunting the ends, wither by fill-in or removal, increases ligation of the poorly- ligated ends.

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