• My NEB
  • Print
  • PDF
  • FAQ: How can ligation efficiency be increased?

    Less than 50% of AflII fragments ligate in a standard 20 microliter reaction containing 100-500 units of T4 DNA ligase. Ligation can be enhanced by using 1 microliter of high concentration T4 DNA ligase. Blunting the ends, wither by fill-in or removal, increases ligation of the poorly- ligated ends.

    Links to this resource

    Products:
    AflII