The buffer must be completely thawed before use. Dilute the 2X stock with dH2O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 µl reaction should contain 5 µl of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH2O.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.