NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017.

FAQ: How should the NEBuffer be used?

The buffer must be completely thawed before use. Dilute the 2X stock with dH2O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 µl reaction should contain 5 µl of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH2O.
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