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  • FAQ: Can Bst DNA Polymerase be used in other NEBuffers?

    Optimal activity is observed in ThermoPol reaction buffer (200% activity). The unit is assayed in a non-optimal buffer taken from the literature. The enzyme exhibits 50% activity in NEBuffers 1 and 3, and 100% activity in buffers 2 and 4. NEBuffers 1-4 must be supplemented with 0.1% Triton X-100 or 100 µg/ml BSA to achieve these values.