FAQ: Is MmeI sensitive to CpG methylation?

MmeI is blocked by overlapping CpG methylation if the middle C, or the 3’ C, is followed by a G, resulting in CpG methylation of C. For example, the sequence TCCGAC, or the sequence TCCRACG, will not be cut by MmeI if CpG modification methylates the underlined Cytosine residue. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE.

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