FAQ: Is MmeI sensitive to CpG methylation?

MmeI is blocked by overlapping CpG methylation if the middle C, or the 3’ C, is followed by a G, resulting in CpG methylation of C. For example, the sequence TCCGAC, or the sequence TCCRACG, will not be cut by MmeI if CpG modification methylates the underlined Cytosine residue.

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