FAQ: What are the solutions/recipes that I need for transforming NEB 5-alpha Competent E. coli (Subcloning Efficiency)?

2% Vegetable peptone (or Tryptone)
0.5% Yeast Extract
10 mM NaCl
2.5 mM KCl
10 mM MgCl2
10 mM MgSO4

SOB + 20 mM Glucose

LB agar:
1% Tryptone
0.5% Yeast Extract
0.17 M NaCl
1.5% Agar

Blue/White Screening:
X-gal 80 µg/ml
IPTG* 0.3 mM
*Omit IPTG for potentially toxic genes