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  • FAQ: If a final concentration of 200 μM each dNTP is called for, how much of the mix should be added to a 100 μl reaction?

    Add 2 μl. Determined by the equation 100 μl x 200 μM = x μl x 10 mM. Converting gives 100 μl x 200 μM = x μl x 10,000 μM. Solving the equation gives x = 2 μl.