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  • FAQ: How much Mass DNA Ladder should I load on a gel?

    You can load 10 µl (0.5 µg) of the Mass DNA ladder on the gel and compare your DNA sample’s intensity to the ladder’s band of nearest size (if the sample is a 2 kb DNA fragment, compare its intensity to the 2 kb band). Load your sample in 10 or 12 µl using the 6X Orange G loading dye provided with the ladder. For a more accurate mass estimation, you can run your sample next to different volumes of the Mass DNA ladder: 

    Band µl Size ng/5 µl ng/10 µl ng/15 µl ng/20 µl
    1 10 kb 80 160 240 320
    2 6 kb 60 120 180 240
    3 4 kb 40 80 120 160
    4 3 kb 25 50 75 100
    5 2 kb 20 40 60 80
    6 1 kb 15 30 45 60
    7 0.5 kb 10 20 30 40