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  • FAQ: Does the E. coli Poly(A) Polymerase work in the M-MuLV reverse transcriptase buffer?

    Yes. Poly(A) polymerase works well in the M-MuLV reverse transcriptase buffer. If what is intended is to carry out the polyadenylation step and then the RT reaction, we recommend to set-up these reactions in two sequential steps. The polyadenylation reaction can be done first using the reverse transcriptase buffer, then the additional components of the RT can be added to carry out the reverse transcription reaction.