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  • FAQ: What primers should I use to sequence the ends of my insert after I clone it into a pMAL vector?

    Use the malE primer (NEB# S1237S) on the 5´ side of the insert. If you want to sequence the 3´ junction, the pUC/M13 primers that bind to the lacZ-a region (e.g. NEB# S1224S) will work.