HomeFAQsDoes the DNA need to be purified after Antarctic Phosphatase treatment?
FAQ: Does the DNA need to be purified after Antarctic Phosphatase treatment?
No. Heat inactivate for 2 minutes at 80°C or 5 minutes at 70°C (or as required to inactivate the restriction enzyme). If the restriction enzyme cannot be heat inactivated, the DNA should be purified prior to ligation.”