HomeFAQsDoes the DNA need to be purified after Antarctic Phosphatase treatment?
FAQ: Does the DNA need to be purified after Antarctic Phosphatase treatment?
No. Heat inactivate for 2 minutes at 80°C or 5 minutes at 70°C (or as required to inactivate the restriction enzyme). If the restriction enzyme cannot be heat inactivated, the DNA should be purified prior to ligation.”
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.