FAQ: Is TaqI Methyltransferase used in any special techniques?

Yes. TaqI Methyltransferase (TCGA*) can be used to create the 8 base recognition site TCGA*TCGA that can be cleaved with DpnI. DpnI recognizes GATC and cleaves when the DNA is methylated. Do not overdigest the DNA because DpnI will cut hemimethylated DNA slowly (TCGATC).