HomeFAQsWhat Poly(U) length can be expected to be incorporated by Poly(U) Polymerase?
FAQ: What Poly(U) length can be expected to be incorporated by Poly(U) Polymerase?
In the standard recommended reaction the tailing length for the Poly(U) polymerase is a function of both accessible primer and UTP concentration. Using a primer where 100% of the population is extended, a typical 30 minute incubation with 0.5 mM UTP will extend all RNA molecules approximately 1,000 bases.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.