FAQ: Is PstI blocked by methylation?

PstI is not blocked by Dam, Dcm or CpG methylation. However PstI is blocked by certain types of methylation sometimes found in plant DNA. Plants often have CAG and/or CTG methylation leading to MTG and/or MAG sites. Where M is 5mC. Pst I is blocked by this type of methylation. This is not the same as CpG methylation (MG as opposed to MTG and MAG). For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Pst I will also not cut AGCTGCAG when methylated by AluI methylase.