PstI is not blocked by Dam, Dcm or CpG methylation. However PstI is blocked by certain types of methylation sometimes found in plant DNA. Plants often have CAG and/or CTG methylation leading to MTG and/or MAG sites. Where M is 5mC. Pst I is blocked by this type of methylation. This is not the same as CpG methylation (MG as opposed to MTG and MAG). For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Pst I will also not cut AGCTGCAG when methylated by AluI methylase.
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