• My NEB
  • Print
  • PDF
  • FAQ: Why do I see products of the wrong size?

    These could be several reasons:
    1. Always do a negative control reaction in the absence of reverse transcriptase.
    2. In cases where the RNA sample is contaminated with genomic DNA, treat with DNase I before cDNA synthesis (5).
    3. Design primers spanning an exon-exon boundary.