- Always do a negative control reaction in the absence of reverse transcriptase.
- In cases where the RNA sample is contaminated with genomic DNA, treat with DNase I before cDNA synthesis (5).
- Design primers spanning an exon-exon boundary.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.