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  • FAQ: At what rate does phi29 DNA Polymerase add nucleotides to a primed single-stranded template?

    The following are phi29 DNA Polymerase rates at different temperatures using a primed single-stranded M13 template(1):
    2280 nt/min at 30°C
    1490 nt/min at 15°C
    290 nt/mn at 4°C

    (1) Soengas, M.S., Gutierrez, M.S. and Salas, M. 1995 J. Mol. Biol.253(4):517-529.