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  • FAQ: Will T4 RNA Ligase 1 ligate single stranded DNA?

    Yes. The reaction is slower than RNA/RNA or RNA/DNA. For example, primers can be ligated onto cDNA (2).
    (2) Troutt, A. B., et al. (1992) Proc. Natl. Acad. Sci. USA 89. pp. 9823-9825.