FAQ: Why isn't TaqαI cutting?

1. It could be inhibited by some mini-prep impurities. Drop-dialyse or ethanol wash.
2. It is blocked by overlapping dam methylation.
3. PCR products can be resistant to cleavage. Purify the DNA.
4. Incubation without BSA results in 50% activity.
5. Incubation at 37°C results in 10% activity.

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