• My NEB
  • Print
  • PDF
  • FAQ: Why is induced protein insoluble (C3010)?

    Check for insolubility - this is important because T7 expression often leads to very high production of protein that can result in the target protein becoming insoluble. Solutions around this are:

    » Induce at lower temperatures (as low as 12 - 15°C overnight)
    » Reduce IPTG concentration to 0.01 - 0.1 mM
    » Induce for less time (as little as 15 minutes)
    » Induce earlier in growth (OD600 = 0.3 or 0.4)