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  • FAQ: Why is induced protein insoluble (C2527)?

    Check for insolubility - this is important because T7 expression often leads to very high production of protein that can result in the target protein becoming insoluble. Solutions around this are:
    • Induce at a lower temperature (as low as 15°C overnight)
    • Reduce IPTG concentration to 40 µM
    • Induce earlier in growth phase (OD600 = 0.3 or 0.4)
    • Test expression in Lemo21(DE3)