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  • FAQ: Which SHuffle strains are resistant to chloramphenicol? Is chloramphenicol required for maintenance of the mini F plasmid?

    Only SHuffle® T7 Express lysY Competent E. coli (NEB #C3030) are resistant to Cam. The Cam resistance is due to the miniF which carries the lysY gene. SHuffle® T7 Express Competent E. coli (NEB #C3029) is sensitive to Cam.

    It is not necessary to add Cam to propagate the miniF. We do not recommend adding Cam to cultures of SHuffle® T7 lysY Competent E. coli (NEB #C3027) or SHuffle® T7 Express lysY Competent E. coli (NEB #C3030). There is one copy of the Cam resistance gene on the miniF therefore standard concentrations of Cam (33 micrograms per ml) may be too high and inhibit growth.