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  • FAQ: Which DNA polymerase do you recommend for amplification of converted DNA?

    EpiMark™ Hot Start Taq DNA Polymerase is a very good choice (NEB #M0490), because no initial activation time is required and the buffer composition is optimized for AT-rich PCR. Hot start DNA polymerases requiring > 2 minutes for activation are not recommended; because the DNA after bisulfite reaction is degraded, heating for > 2 extra minutes at 95°C will cause severe degradation.