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  • FAQ: When should I choose the High Fidelity (HF) version of the enzyme?

    The HF version of the enzyme has the same cleavage specificity as the wild type enzyme, and should be chosen if star activity is a concern or if the recommended buffer is more convenient for double digestion or other multi-step protocol. There is no disadvantage to using the HF version. Learn more about High Fidelity Restriction Enzymes here.