The HF version of the enzyme has the same cleavage
specificity as the wild type enzyme, and should be chosen if star
activity is a concern or if the recommended buffer, CutSmart®, is more
convenient for double digestion or other another multi-step protocol. In
some instances, HF enzymes may have different heat inactivation
temperature (or time), salt tolerance and (rarely) methylation
Learn more about High Fidelity Restriction Enzymes.