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  • FAQ: When should Bst DNA Polymerase be the enzyme of choice?

    Bst DNA Polymerase is good at strand displacement. It fills a void between thermophilic and mesophilic polymerases. The temperature optimum of 60-65°C is higher than DNA Polymerase I, Large (Klenow) Fragment (NEB #M0210) and lower than VentR DNA Polymerase (NEB #M0254), two other strand displacing polymerases. This gives researchers a wider range of reaction conditions to optimize strand displacement and primer annealing. This is useful in the design of sequencing strategies as well as isothermal amplification technologies. The elevated reaction temperature facilitates sequencing through GC rich regions.

    NEB's Selection of Bst DNA Polymerase-based Products for Isothermal DNA Amplification
      5′ to 3′
    EXO ACTIVITY
    AMPLIFICATION
    SPEED
    ROOM
    TEMPERATURE
    SETUP
    REVERSE
    TRANSCRIPTASE
    ACTIVITY
    INHIBITOR
    TOLERANCE
    APPLICATIONS
    Bst DNA Polymerase,
    Full Length

    **

    N/A

    N/A

    N/A

    *
    Nick translation reactions at elevated temperatures
    Bst DNA Polymerase,
    Large Fragment

    N/A

    *

    N/A

    *

    *
    General strand-displacement reactions, original polymerase for LAMP and other diagnostic amplifications
    Bst 2.0
    DNA Polymerase
     
    N/A

    **
     
    N/A

    **

    **
    Improved LAMP, SDA, and other amplification reactions
    Bst 2.0 WarmStart
    DNA Polymerase
     
    N/A

    **

    ***

    **

    **
    Consistent, room-temperature, and high-throughput amplification assays
    Bst 3.0
    DNA Polymerase
     
    N/A

    ***

    **

    ***

    ***
    Fastest, most robust LAMP and RT-LAMP reactions. High reverse transcriptase activity up to 72°C

    ***   Optimal, recommended product for selected application
     **   Works well for selected application
      *    Will perform selected application, but is not recommended
    N/A  Not applicable to this application