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  • FAQ: When do I need to use the E. coli ligase for Fragmentase?

    If the DNA fragments created by dsDNA Fragmenase will be exposed to a strand-displacing enzyme such as Klenow exo-, before being treated with a ligase, then E. coli DNA Ligase for Fragmentase should be used. If the fragmented DNA will be used for sample preparation for Illumina sequencing then the E. coli DNA Ligase for Fragmentase should be used.