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  • FAQ: What is the composition of the T4 DNA Ligase Reaction Buffer?

    1X T4 DNA Ligase Reaction Buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM Dithiothreitol 1 mM ATP, pH 7.5 @ 25°C. Supplied as a 10X concentrated stock.