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  • FAQ: What is the activity of T7 Endonuclease I at different reaction temperatures?

    0°C: some nicking
    25°C: some nicking
    37°C: 10,000 units/ml
    45°C: 20-40,000 units/ml
    65°C: 10-20,000 units/ml
    80°C: 6,000 units/ml
    90°C: 500 units/ml
    The literature reports that the enzyme loses its ability to detect insertions or deletions at higher temperatures.