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  • FAQ: What is a common cause of β-Agarase I reaction failure?

    Working at the wrong temperature. β-Agarase I is quickly inactivated at temperatures above 45°C. The low melting point agarose must be melted at 65°C then equilibrated at 42°C. Therefore, when working with large volumes, be sure to leave ample time for molten agar to equilibrate to 42°C. At lower temperatures the agarose starts to solidify making it resistant to digestion.