FAQ: What are the solutions/recipes (C2986)?


SOB:
2% Vegetable peptone (or Tryptone)
0.5% Yeast Extract
10 mM NaCl
2.5 mM KCl
10 mM MgCl2
10 mM MgSO4 

SOC:
SOB + 20 mM Glucose 

LB agar:
1% Tryptone
0.5% Yeast Extract
0.17 M NaCl
1.5% Agar 

Blue/White Screening:
X-gal 80 µg/ml 
IPTG* 0.3 mM 

*Omit IPTG for potentially toxic genes