• My NEB
  • Print
  • PDF
  • FAQ: What are the shortest overlaps that can be used with this assembly method?

    Productive assembly has been shown for DNA fragments with as little as a 12 bp overlap, however, it depends on the GC content of the overlap. We recommend using 15 bp overlaps, or more, for dsDNA assembly with a Tm ≥ 48°C (AT pair = 2°C and GC pair = 4°C).