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  • FAQ: What are the differences between the reaction products of fragmentation with the NEBNext® Magnesium RNA Fragmentation Module and the NEBNext® RNase III RNA Fragmentation Module?

    The NEBNext RNase III RNA Fragmentation Module uses a riboendonuclease that cleaves long double stranded RNAs into RNA fragments with 5' phosphate and 3' hydroxyl termini. The NEBNext® Magnesium RNA Fragmentation module uses a divalent metal ion (Mg++) and heat to fragment RNA; creating RNA fragments with 5’ hydroxyl and 3’phosphate termini.