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  • FAQ: What are the advantages or disadvantages of Crimson Taq DNA Polymerase?

    The Crimson Taq Reaction Buffer formulation offers three convenient features. First, the 5X Crimson Taq Reaction Buffer contains a red dye, which serves as a visual indicator of homogeneous reaction setup. Second, the 5X Crimson Taq Reaction Buffer contains a density reagent Dextran, which allows direct loading of PCR products on a gel. Third, the trace amount of red dye in Crimson Taq Reaction Buffer works as a gel tracking dye (about 10 bp) during electrophoresis.

    If the PCR products will be analyzed by absorbance or fluorescence excitation, acid red (λmax= 510 nm) may interfere with the assay; therefore Standard Taq Reaction Buffer is recommended.