FAQ: What are the advantages of this method compared to traditional cloning methods?

NEBuilder HiFi: the next generation of DNA Assembly

In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.

Gibson Assembly allows insertion of one or more DNA fragments into virtually any position of the linearized vector and does not rely on the presence of restriction sites within a particular sequence to be synthesized or cloned. Therefore, the user has complete control over what is assembled and insertion of unwanted additional sequence, often used to facilitate the manipulation of multiple DNA sequences, can be avoided. Furthermore, the Gibson Assembly method is fast relative to standard restriction enzyme-based cloning. Lastly, a greater number of DNA fragments can be joined in a single reaction with greater efficiency than conventional methods.