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  • FAQ: What are the advantages of the IMPACT System?

    • Single column purification - no additional steps to remove the affinity tag
    • Yields native amino acid sequence
    • Fusion of a target protein to either the C-terminus or N-terminus of the intein tag
    • Proteases are NOT required to remove the affinity tag from the target protein
    • Use of either thiol reagent or pH and temperature shift to induce on-column cleavage
    • Isolation of proteins with or without an N-terminal methionine residue
    • Production of proteins possessing an N-terminal cysteine and/or C-terminal thioester for use in protein labeling, ligation and cyclization [See Part 6: Applications: Protein ligation and labeling]
    • T7 Promoter for high-level expression and tight regulation of transcription
    • Protein semisynthesis - The ability to create a thioester at the C-terminus for ligation with a N-terminal containing cysteine peptide or tag [See Part 6: Applications: Protein ligation and labeling]
    • The ability to label the C-terminus of the target protein [See Part 6: Applications: Protein ligation and labeling]
    • IMPACT vectors pTYB21 (NEB #N6709) and pTYB22 (NEB #N6710) contain a MCS that is compatible with other NEB expression vectors.