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  • FAQ: Is this method applicable to the assembly of repetitive sequences?

    Yes. However, one must ensure that each DNA fragment includes a unique overlap so that the sequences may anneal and are properly assembled. The repetitive sequence can also be internalized in the first stage of a two-stage assembly strategy. If having repetitive sequences at the ends of each fragment is unavoidable, the correct DNA assembly may be produced, albeit at lower efficiency than other, unintended assemblies.