FAQ: Is the DNA yield lower using dam-/dcm- strain (C2925)?

No.

Plasmid pUC19 was transformed into dam-/dcm- competent E.coli (NEB #C2925H) and NEB 5-alpha F´ Iq competent E.coli (NEB #C2992H), and plasmid was purified side by side. No difference between these two strains for the DNA quality or yield existed.

Plasmid pBIND was also transformed into dam-/dcm- competent E.coli (NEB #C2925H) and NEB 10-beta competent E.coli (NEB #C3019H), and plasmid was purified side by side. No difference between these two strains for the DNA quality or yield existed.

We recommend inoculating freshly grown colonies for plasmid propagation.