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  • FAQ: Is the DNA yield lower using dam-/dcm- strain (C2925)?

    No.

    Plasmid pUC19 was transformed into dam-/dcm- competent E.coli (NEB #C2925H) and NEB 5-alpha F´ Iq competent E.coli (NEB #C2992H), and plasmid was purified side by side. No difference between these two strains for the DNA quality or yield existed.

    Plasmid pBIND was also transformed into dam-/dcm- competent E.coli (NEB #C2925H) and NEB 10-beta competent E.coli (NEB #C3019H), and plasmid was purified side by side. No difference between these two strains for the DNA quality or yield existed.

    We recommend inoculating freshly grown colonies for plasmid propagation.