Endoproteinase GluC is supplied in one 50ug vial. To get the most use out the enzyme, we make the following recommendation: Add 500 ul H2O to the lyophilized Endoproteinase GluC and aliquot 50 ul in 10 tubes. Freeze the tubes that aren't being used immediately. We freeze Endoproteinase GluC stocks at -80°C for long term storage. The smaller the reconstitution volume, the higher chance of autolysis, so any sample reconstituted in a small volume should be used immediately. Repeated freeze/thawing is not recommended.