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  • FAQ: Is T7 RNA Polymerase an enzyme of choice for making high specific activity labeled probes?

    Yes, SP6 RNA Polymerase (NEB# M0207), T7 RNA Polymerase, and the HiScribe RNAi Transcription Kit (NEB# E2000) can be used to make RNA probes. Increased levels of detection in nucleic acid hybridization can be obtained due to the greater stability of RNA: DNA hybrids with respect to DNA: DNA hybrids (1). The NEBlot Kit (NEB# N1500) can be used for making high specific activity DNA probes using random primers. Klenow Fragment (3'→5' exo-) (NEB# M0212) can be used with the same procedure and a specific primer.
    (1) Zinn, K et al. (1983) Cell 34, 865-879.