FAQ: Is star activity a problem for TaqαI?

Yes. Conditions that should be avoided include low salt concentration, high glycerol concentration, high enzyme concentration, long reaction time, the addition of organic solvents (Gene 65: 149-165 (1988) Barany, F. ) and the addition of Mn2+ (Biochemistry 34: 2276-2283 (1995) Coa, W. Mayer, A.N., Barany, F.).

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