FAQ: Is SacII inhibited by salt?

Moderate levels of salt can inhibit SacII. DNA preperations can have high salt concentrations. If the volume of DNA added to the reaction is a high percentage of the total volume, salt can inhibit cutting. Increasing the reaction volume may help. The DNA may need to be ethanol precipitated and washed with 70% ethanol to reduce the salt. Drop dialysis is the most effective way of reducing the salt.

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